Transformation of cells from the normal human amnion into established strains.
نویسندگان
چکیده
Previous communications from this laboratory have reported the cultivation and poliovirus in fection of cells from the normal human amnion (11), the cytological changes associated with polio virus infection (3), and the continuous cultivation of a strain of cells (5). The present paper describes the transformation of cells from four normal human amnions into seven established, rapidly growing strains. These observations were made over a period of 8-14 months.
منابع مشابه
Preparation and cultivation of primary human amnion cells.
Human amnion tissue is readily available to most laboratories as a source of cells for tissue culture studies. It has proved to be a useful tool in the field of virus research, and it offers impor tant potentials in the field of cancer research as a source of normal human cells easily obtained in primary cultivation. Many investigators have been concerned with the problem of malignant propertie...
متن کاملExpression of Recombinant Coagulation Factor IX in Human Amniotic Membrane-derived Mesenchymal Stem Cells: A New Strategy to Gene Therapy of Hemophilia B
Background: Hemophilia B is an X-linked hereditary disorder of blood coagulation system which is caused by factor IX (FIX) deficiency. Factor IX is a plasma glycoprotein that participates in the coagulation process leading to the generation of fibrin. Replacement of factor IX with plasma-derived or recombinant factor IX is the conventional treatment for hemophilia B to raise the factor IX le...
متن کاملEffect of human amnion-derived multipotent progenitor cells on hematopoietic recovery after total body irradiation in C57BL/6 mice
Background: The hematopoietic system is sensitive to the adverse effects of ionizing radiation. Cellular therapies utilizing mesenchymal stem cells or vascular endothelial cells have been explored as potential countermeasures for radiation hematopoietic injuries. We investigated cells cultured from amnion ...
متن کاملHuman Amniotic Fluid Stem Cells: General Characteristics and Potential Therapeutic Applications
Introduction: Amniotic fluid contains a mixture of different cell types sloughed from the fetal skin, respiratory, alimentary and urogenital tracts, as well as the amnion membrane. As amniotic fluid develops prior to the process of gastrulation, many cells found in its heterogeneous population do not undergo lineage specialization. Therefore, amniotic fluid-derived mesenchymal stem cells (AF-MS...
متن کاملOptimization of Agrobacterium-mediated transformation in oyster mushroom (Pleurotus ostreatus) by vector containing human pro-insulin gene
Transferring foreign genes into mushroom mediated by Agrobacterium tumefaciens is a standard technique in genetic engineering. Recombinant human insulin has been greatly used in the treatment of type I diabetes. The production of edible mushroom derived insulin should facilitate oral delivery. In this study we used the Agrobacterium tumefaciens mediated transformation method for the transfer an...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Cancer research
دوره 17 11 شماره
صفحات -
تاریخ انتشار 1957